To prepare and dispense 10 ml Normal Saline.

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Object – To prepare and dispense 10 ml Normal Saline.

Reference – Gaud R.S. and Gupta G.D., Practical Pharmaceutics, CBS Publishers & Distributors Pvt. Ltd., 1st Edition 2002, Reprint 2019, Page no. .

Requirements –

  • Chemicals – Sodium Chloride, Water for Injection.
  • Glassware – Beaker, Autoclave, Funnel, Volumetric Flask, Glass Rod.

Theory –

Saline solution is a mixture of salt and water. The normal saline solution contains 0.9 percent sodium chloride (salt), which is similar to the sodium concentration in blood and tears. Saline solution is usually called normal saline, but it’s sometimes referred to as physiological isotonic saline. It contains no antimicrobial agent; as it is a large volume and single-dose parenteral preparation. Sodium Chloride Injection contains not less than 0.85% w/v and more than 0.95% w/v of sodium chloride.

Formula Table:

S. No.IngredientsQuantity GivenQuantity Taken
1.Sodium Chloride0.9 gm0.09 gm
2.Water for injection100 ml10 ml


  1. Accurately weigh all the ingredients.
  2. Add sodium chloride in a suitable sterile container and add a sufficient quantity of water for injection to make the required volume. Stir to dissolve.
  3. If the solution is clear or not, filter the solution by using sintered glass filter.
  4. Fill the clear filtrate solution into the stainless-steel pressure tank and aseptically pass-through membrane filter of 0.22 im.
  5. Fill the solution aseptically into a sterilized container and close with a sterilized rubber closure and seal it with aluminium cap.
  6. Check the leakage and reject the leaking container.
  7. Examine the solution critically for particulate matter.
  8. Then sterilized by autoclave at temperature121°C and pressure 15 lbs /sq. inch for 20 mins.
  9. Switch off the autoclave. Allow it to cool and the pressure to come to zero, then remove containers.
  10. Polish the container and re-examine for the particulate matter and leakage.
  11. Label it and submit.

Category: Fluid and Electrolyte replenisher.

Storage: It should be stored in a cool dark place, protect from light.

Use: Fluid and Electrolyte replenisher.

Result: 10 ml Normal saline had been prepared and dispensed.


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