To prepare the sub-culturing of bacteria and fungus.

0
503
WhatsApp D. Pharma Group Join Now
Telegram D. Pharma Group Join Now
Telegram Group Latest Pharma Jobs Join Now
Telegram B. Pharma Group Join Now
Telegram Medicine Update Group Join Now
WhatsApp B. Pharma/ GPAT Channel Join Now

Aim – To prepare the sub-culturing of bacteria and fungus.

Requirement – Mixed nutrient agar streaks, Nutrient agar slants or nutrient agar plates, Inoculating loop, Wax marking pencil, microscope

Theory – Following streak plate, pour plate, or spread plate incubation and examination of the appearance of the distinct, well-separated colonies, the next step is to subculture some of the cells from one of the colonies to separate agar plates or nutrient agar slants with a sterilised needle or loop for further analysis and use. One species known as a pure culture or stock culture is represented by each of these new cultures, which is the expansion of that species. Transferring microorganisms from one medium to another or from one parent growth source to a fresh one is referred to as subculturing.

Procedure –

image 16
To prepare the sub-culturing of bacteria and fungus. 2
  1. With a wax pencil label the nutrient agar slants and agar plates as bacteria A & B. Sterilize the inoculating loop by holding it in the hottest portion of the Bunsen burner flame.
  2. Flame until entire wire become red hot.
  3. Allow the loop to cool for a few seconds or cool it by dipping in a fresh agar plate.
  4. Touch the tip of the loop to the surface of a selected discrete colony or the agar streak plate or the pour plate.
  5. Remove the plug of the agar slants, grasp the plug with thee little finger of the left hand and pass the neck tube rapidly over the Bunsen burner flame. Inset the loop into the subculture tube rapidly over the Bunsen burner flame. Inset the loop into the subculture tube and inoculate it lightly over the hardened surface in a straight or zig zag line and recap tube.
  6. Reflame the inoculating loop/ needle to destroy existing organism.
  7. Incubate the culture for 48-72hrs.

Result – The sub-culturing of bacteria and fungus was prepared.

LEAVE A REPLY

Please enter your comment!
Please enter your name here